Anonymous at Fri, 17 May 2024 02:23:21 UTC No. 16179232

That's smallpox.

Anonymous at Fri, 17 May 2024 02:25:19 UTC No. 16179236

>>16179216
i have also absolute no glue. maybe a microscope image would help ?

Anonymous at Fri, 17 May 2024 02:37:03 UTC No. 16179243

>>16179236
hi this is the op!!! here’s a pic under I believe 400x! might be under oil too, sorry I took these forever ago but I need to identify them now :,) thank you for offering help!

Anonymous at Fri, 17 May 2024 02:41:36 UTC No. 16179245

>>16179243
can you try heating a live culture and seeing if it survives? I'm an MD student so everything I know is path related and broad strokes. Cereus should regrow after high heat due to spore formation.

Anonymous at Fri, 17 May 2024 02:42:38 UTC No. 16179246

>>16179245
>>16179243
not sure if badius and megatarium are also spore forming and I don't want to look it up so i might just be an idiot here.

Anonymous at Fri, 17 May 2024 02:42:57 UTC No. 16179247

>>16179216
>honestly trying to discuss science on /sci/
sweet summer child
i'd help, but am an electrical engineer with no experience in biology

Anonymous at Fri, 17 May 2024 02:46:29 UTC No. 16179249

>>16179243
i am computer science guy. so like i said i have no glue. but it looks like megatarium.

i guess megatarium builds these chains ?

Anonymous at Fri, 17 May 2024 02:55:48 UTC No. 16179257

>>16179249
MD student anon here
gram positive bacilli in chains are characteristic for Bacillus species

Anonymous at Fri, 17 May 2024 02:57:17 UTC No. 16179260

>>16179257
also my previous suggestion of high heat. wouldn't work as all Bacillus spp are spore forming. Duh.

Anonymous at Fri, 17 May 2024 02:59:57 UTC No. 16179265

>>16179257
but when i google for badius and cereus then the images are different
when i google for megatarium the it fits for me
purely based on the image

Anonymous at Fri, 17 May 2024 03:03:24 UTC No. 16179269

>>16179265
do you mean the gram stain or the colonies? Because all Bacillus spp. are gram positive cocci that form chains

Anonymous at Fri, 17 May 2024 03:06:24 UTC No. 16179272

>>16179269
>cocci
Bacilli*

Anonymous at Fri, 17 May 2024 03:15:23 UTC No. 16179279

>>16179216
Having compared the three species based on images, I'd say it's certainly not badius. The remainder possibilities seem to be too similar (at least cell-shape and colony-formation wise), so I'm following two works that comment about cereus and megatarium colony morphologies:
https://www.cabidigitallibrary.org/doi/pdf/10.5555/20210260274
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273213/

My guess based on the colony morphology you pictured and described would be megatarium.

Anonymous at Fri, 17 May 2024 03:36:33 UTC No. 16179308

>>16179279
op here again! i agree, i basically ruled out badius as soon as i saw some colonies of it haha also i think its lipase negative??? not 100% sure… why is it so hard to find this info sometimes??? anyways! i totally agree w you based on everything I’ve seen, according to my professor b. cereus doesn’t grow at all or very little on MSA (mannitol salt agar) and i had some pretty good growth which is far more characteristic of b. mega! supposedly b. cereus is negative for lipase too but ill have to fact check that one…

i’ve attached a very early photo of the colonies on agar as i feel they more closely resemble b. mega here :)

Anonymous at Fri, 17 May 2024 03:38:15 UTC No. 16179315

>homework thread

>>16179216
read the rules before posting, shit for brains

Anonymous at Fri, 17 May 2024 03:45:23 UTC No. 16179326

>>16179315
hi first of all im sorry if i used this board incorrectly ive never used 4chan before. and secondly, i’m asking for research purposes in a lab setting.

Anonymous at Fri, 17 May 2024 03:56:57 UTC No. 16179332

>>16179216
>hi this is my first time using 4chan
ahhh fresh meat, welcome to the dark side

Anonymous at Fri, 17 May 2024 04:08:59 UTC No. 16179340

>>16179332
hasn’t been too bad so far! i’m staying on this board for a reason LMFAOOO

Anonymous at Fri, 17 May 2024 04:10:44 UTC No. 16179341

>>16179308
No problem. I study biology but I'm not too much into bacteria, so I learned a tiny bit researching colony identification. Although at this point given the expected diversity and HGT, I wonder how useful species identification is for those groups.

>>16179315
I'd take this "homework thread" that actually discusses biology any day over the hundreds of "le evolution" or "le races" bait posts. A shame how /an/ has become a better place for biology than here, although it's expected since most people in this board are actually scientifically illiterate and just looking for bait feeding.

Anonymous at Fri, 17 May 2024 04:11:01 UTC No. 16179342

>>16179326
this isnt reddit as long you dont post childporn its ok

Anonymous at Fri, 17 May 2024 04:38:03 UTC No. 16179365

>>16179216
oxidase? also what agar is that and how was it incubated (aerobic or anaerobic)

Anonymous at Fri, 17 May 2024 04:53:11 UTC No. 16179369

>>16179365
i used nutrient agar plates that i believe support both anaerobic and aerobic bacteria! the first photo in the thread shows a large plate incubated at 25C whereas the most recent shows a smaller plate incubated at 37C if that helps at all. i unfortunately did not have the opportunity to run an oxidase test but i can absolutely see how that would help :(
sorry if these answers are a little vague, im not super well versed in the realm of incubation (my partner is wayyy better at that) as i am more curious about the results haha

if you’re curious about any results for other tests i mentioned, I have pictures i can send for those :)

Anonymous at Fri, 17 May 2024 04:58:53 UTC No. 16179373

>>16179365
^ wait I think i’m actually losing it i think we definitely did aerobic incubation sorry i had a massive brain fart i’m exhausted :p

Anonymous at Fri, 17 May 2024 05:20:40 UTC No. 16179387

you sure it's a bacillus sp? gram positive rod that's catalyse pos (and would be oxidase neg) that grows well at 25 could ez be listeria monocytogenes. Put it on Oxford agar

Anonymous at Fri, 17 May 2024 05:40:07 UTC No. 16179405

You should see how large your biggest colonies are. B. megaterium gets pretty big for a bacterium.

If you have access to MYP, it's a surefire way of seeing whether you have cereus since it'll turn pink. You should get hemolysis on a blood plate as well.

B. cereus and B. megaterium like to grow at around 30C, but megaterium is a bit more tolerant towards higher temperatures. You likely have a 42C incubator in your lab. Try to grow it on something nonselective at that temperature if you don't have access to blood agar or MYP (or PEMBA, but that shit is expensive).
t. Food safety microbiologist.

Also, B. megaterium got reclassified into some gay genus called Priestia. You can ackshully your professor or TA if you want to give them a hard time.

Anonymous at Fri, 17 May 2024 05:47:17 UTC No. 16179409

>>16179405
Also I know nothing about B. badius. Sorry OP. It exists but idk how to differentiate it bar MYP/PEMBA from experience. Not a lot of client samples pop for it.

Anonymous at Fri, 17 May 2024 06:17:33 UTC No. 16179437

>>16179326
>incorrectly ive never used 4chan before
Just lurk moar

Anonymous at Fri, 17 May 2024 06:26:55 UTC No. 16179441

>>16179341
>A shame how /an/ has become a better place for biology than here
You say that as if we have an easy time discussing non-biology topics here in the midst of all the spam and bait threads.

Anonymous at Fri, 17 May 2024 12:34:38 UTC No. 16179743

>>16179387
Could look at live colonies to see if it's motile for listeria

Anonymous at Fri, 17 May 2024 12:40:56 UTC No. 16179752

>>16179247
Saaaaar can you help me select with moving my 4QPSK modulator over to VHDL / Xilinx ? I need to escape a bottleneck here.

Anonymous at Sat, 18 May 2024 04:19:52 UTC No. 16180970

>>16179216
Can't you figure this out by doing pcr?

Anonymous at Sat, 18 May 2024 04:21:31 UTC No. 16180972

>>16179441
You're lucky to even have some key words popping up, although I do agree the situation is far from good to anyone.

Anonymous at Sat, 18 May 2024 05:03:18 UTC No. 16181012

>>16180970
Doing PCR to find out if a bacteria is gram positive is just overkill.

>>16180972
>You're lucky to even have some key words popping up
How so?

Anonymous at Sat, 18 May 2024 05:10:37 UTC No. 16181028

>>16181012
He's looking for a species ID. To tell gram, he just needs to do some staining.

Anonymous at Sat, 18 May 2024 15:13:42 UTC No. 16181552

>>16179243
>>16179269
they look fucking discusting

Anonymous at Sat, 18 May 2024 17:37:35 UTC No. 16181760

>>16181552
So you are you faggot but you don't here anyone being a bitch about it

Anonymous at Sun, 19 May 2024 10:39:54 UTC No. 16182598

bump for further interest

Anonymous at Mon, 20 May 2024 01:15:55 UTC No. 16183667

To identify what it is you need to make agar/agarose plates with antibiotics that would kill specifically certain types of the bacteria and not others. You would have to try and grow colonies of this bacterial sample on those plates and whichever it grows on you can rule that out (as it didnt die) and whichever it dies on (it should conveniently be just 1 plate if you do your research right and can produce those antibiotics needed that dont have an overlap in which type of bacteria it kills). If you cannot get certain antibiotics and there are overlaps then you will have to figure out a way with this method to identify what you need. Also remember to have positive and negative controls.

Anonymous at Mon, 20 May 2024 01:19:45 UTC No. 16183673

>>16183667
Sorry I forgot to add: whichever it dies on most likely is that bacteria or something adjacent to if (do your research for that). This will require research and getting materials on your part but thats just a part of the process. This is how I would go about doing what you are doing.