🧵 Which cells does mrna and viral vector vaccines target/enter?
Anonymous at Tue, 25 Jun 2024 17:25:20 UTC No. 16253000
I heard both dendtritic cells and muscle cells, so which is it?
Anonymous at Tue, 25 Jun 2024 18:07:25 UTC No. 16253098
Shit, is being vaxxed killing my gains?
Anonymous at Tue, 25 Jun 2024 19:07:37 UTC No. 16253215
>>16253000
It can enter any type of cell. Most of the damage is neuromuscular and endothelial though. This already covers three of the basic histological categories.
Anonymous at Tue, 25 Jun 2024 19:12:45 UTC No. 16253228
>>16253215
couldn't that cause problems since it's non replicating?
Anonymous at Tue, 25 Jun 2024 19:14:20 UTC No. 16253231
>>16253228
I mean non dividing
Anonymous at Tue, 25 Jun 2024 19:41:08 UTC No. 16253310
>>16253228
It causes a lot of problems. My mobility is impaired since I took Pfizer in 2021 and I have wandering inflammations in various regions of my body.
Anonymous at Tue, 25 Jun 2024 19:47:30 UTC No. 16253325
>>16253000
Revelant but John Green video about TB/ how the white death is the worst thing we've ever had. Worthwhile mention that we came out of Africa.
https://m.youtube.com/watch?v=GFLb5
Anonymous at Wed, 26 Jun 2024 00:30:42 UTC No. 16253994
>>16253310
>mobility is impaired
How so?
Anonymous at Wed, 26 Jun 2024 12:21:56 UTC No. 16254637
>>16253310
I'm sorry to hear it. I have taken the two different mRNAs and I'm fine. Everyone I know is also fine after several shots, but one person is apparently also affected a bit more permanently, but that person is elderly.
Anonymous at Wed, 26 Jun 2024 13:20:16 UTC No. 16254714
>>16253000
bump
Anonymous at Wed, 26 Jun 2024 14:42:07 UTC No. 16254815
>>16253000
>on DNA integration:
>at least 5 different mechanisms for that DNA-RNA-protein combination to take that DNA into the nucleus of your cells
https://arkmedic.substack.com/p/5-w
>case studies and singular results, some post infection and some in vitro. Reverse integration seems to be possible.
https://pubmed.ncbi.nlm.nih.gov/339
>Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues
https://www.mdpi.com/1467-3045/44/3
>Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line
https://www.biorxiv.org/content/10.
>SARS-CoV-2 RNA reverse-transcribed and integrated into the human genome
https://www.ncbi.nlm.nih.gov/pmc/ar
>Adenoviral Vector DNA- and SARS-CoV-2 mRNA-Based Covid-19 Vaccines: Possible Integration into the Human Genome - Are Adenoviral Genes Expressed in Vector-based Vaccines?
>the vax spike protein itself contains a Nuclear Localisation Signal (NLS), so even without the SV40 enhacer (another NLS), DNA can be transported to the nucleus
>Coincidentally the NLS is also the furin cleavage site.
>NLS is also a peptide sequence. The DNA forms bonds to proteins that have an NLS and piggybacks on it into the nucleus (as seen in Cytoplasmic transport and nuclear import of plasmid DNA; Bai et al)
>remember spike protein (with NLS) is produced in the same cytoplasm as that plasmid
https://www.ncbi.nlm.nih.gov/pmc/ar
>Nuclear translocation of spike mRNA and protein is a novel feature of SARS-CoV-2
https://www.theqtree.com/2023/05/01
>Pfizer and Moderna Vaccines Both Contain the PRRARSV Key to the Cell Nucleus
Anonymous at Wed, 26 Jun 2024 14:44:30 UTC No. 16254819
>>16254815
>plasmid integration:
https://www.ncbi.nlm.nih.gov/pmc/ar
>Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase
https://www.ncbi.nlm.nih.gov/pmc/ar
>Engineering of the current nucleoside-modified mRNA-LNP vaccines against SARS-CoV-2
https://www.ncbi.nlm.nih.gov/pmc/ar
>Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase
>the LINE1 pathway
https://www.pnas.org/doi/10.1073/pn
>Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues
Anonymous at Wed, 26 Jun 2024 16:47:41 UTC No. 16254966
>>16254815
>>16254819
tl;dr
qrd?
Anonymous at Wed, 26 Jun 2024 18:22:27 UTC No. 16255102
>>16253098
Your only gains will be turbo cancers.
Anonymous at Wed, 26 Jun 2024 18:41:33 UTC No. 16255123
>>16255102
thanks for the bump /pol/tard
Anonymous at Wed, 26 Jun 2024 19:28:13 UTC No. 16255188
>>16254966
there are at least 5 different mechanisms for the mRNA vax to take that foreign DNA into the nucleus of your cells, any cell
https://arkmedic.substack.com/p/5-w
>>1: The Lipid Nanoparticles
>if the LNPs (or lipofectamine or any other cationic lipid particle) hang around for a few hours they will transfect (bring DNA into) the nucleus of any cell that it is in contact with.
>>2: Linearised plasmid DNA
>the plasmid is the circular loop of DNA that is used to transfect the E.Coli
>n the vax case it's a subproduct of the manufacturing process
https://www.nature.com/articles/s41
High spontaneous integration rates of end-modified linear DNAs upon mammalian cell transfection
>>3: The SV40 enhancer
>A gene enhancer is a switch that ramps up the production of gene product (protein) that it is affiliated with. In the case of SV40 the enhancer will essentially turn a gene on and never switch it off.
>>4. Spike protein nuclear localisation signal
>the spike protein (not the RNA or DNA) contains a special peptide sequence which acts as a nuclear transporter of any DNA that is attached to it.
>there is a nuclear localisation signal (NLS) in the spike protein. It should have been removed when they “created the vaccine in record time”
>The nuclear localisation signal (NLS) is the sequence PRRARSV. It’s the same sequence as the Furin Cleavage Site.
>>5. Open Reading Frame in the PolyA tail
>The “PolyA tail” is the end cap on an mRNA sequence.
>Theoretically - if that sequence were to “read through” the stop codons ahead of it, could carry anything into the nucleus.
>in Pfizer and Moderna “mRNA vaccines”,they use pseudouridine, which is known to carry a risk of precisely this event happening.
Anonymous at Wed, 26 Jun 2024 20:04:01 UTC No. 16255259
>>16255188
Barely understood any of it but will look into it later, thanks :).
Barkon at Wed, 26 Jun 2024 20:33:03 UTC No. 16255327
Mouf, now
Anonymous at Thu, 27 Jun 2024 13:31:33 UTC No. 16256601
bump
Anonymous at Thu, 27 Jun 2024 20:34:49 UTC No. 16257199
>>16253000
both probably